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flow cytometry attune nxt v6  (Thermo Fisher)


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    Thermo Fisher flow cytometry attune nxt v6
    Flow Cytometry Attune Nxt V6, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flow+cytometry+attune+nxt+v6/attune+nxt+flow+cytometer/pm38539048-96-4-9
    Average 90 stars, based on 1 article reviews
    flow cytometry attune nxt v6 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Staining:

    Article Title: Acute inhalation of tungsten particles results in early signs of cardiac injury.
    Article Snippet: Cells were washed and resuspended in staining buffer (1 x dPBS; 10 mM sodium azide, 5% FBS) before being analyzed by flow cytometry (Attune NxT V6, ThermoFisher Scientific).

    Article Title: A whale of a tale: whale cells evade the driving mechanism for hexavalent chromium-induced chromosome instability.
    Article Snippet: Chromosome instability, a hallmark of lung cancer, is a driving mechanism for hexavalent chromium [Cr(VI)] carcinogenesis in humans.. Cr(VI) induces structural and numerical chromosome instability in human lung cells by inducing DNA double-strand breaks and inhibiting homologous recombination repair and causing spindle assembly checkpoint (SAC) bypass and centrosome amplification.. Great whales are long-lived species with long-term exposures to Cr(VI) and accumulate Cr in their tissue, but exhibit a low incidence of cancer.

    Article Title: Inhalation of Tungsten Metal Particulates Alters the Lung and Bone Microenvironments Following Acute Exposure.
    Article Snippet: ROS was measured by flow cytometry (Attune NxT V6) using the indicator dye 20,70- dichlorodiydrofluorescein diacetate (H2DCFDA; Thermofisher Scientific [eg, 495 nm/em: 527 nm], which is reduced to 20,70- dichloroflurescein (DCF) in the presence of ROS, following the manufacturer’s protocol.

    Article Title: Inhalation of Tungsten Metal Particulates Alters the Lung and Bone Microenvironments Following Acute Exposure.
    Article Snippet: Cells were resuspended in 300 ml of staining buffer (1 dPBS, 10 mM sodium azide, 5% FBS) before being analyzed by flow cytometry (Attune NxT V6, Thermofisher Scientific).

    Flow Cytometry:

    Article Title: Acute inhalation of tungsten particles results in early signs of cardiac injury.
    Article Snippet: Cells were washed and resuspended in staining buffer (1 x dPBS; 10 mM sodium azide, 5% FBS) before being analyzed by flow cytometry (Attune NxT V6, ThermoFisher Scientific).

    Article Title: A whale of a tale: whale cells evade the driving mechanism for hexavalent chromium-induced chromosome instability.
    Article Snippet: Chromosome instability, a hallmark of lung cancer, is a driving mechanism for hexavalent chromium [Cr(VI)] carcinogenesis in humans.. Cr(VI) induces structural and numerical chromosome instability in human lung cells by inducing DNA double-strand breaks and inhibiting homologous recombination repair and causing spindle assembly checkpoint (SAC) bypass and centrosome amplification.. Great whales are long-lived species with long-term exposures to Cr(VI) and accumulate Cr in their tissue, but exhibit a low incidence of cancer.

    Article Title: Inhalation of Tungsten Metal Particulates Alters the Lung and Bone Microenvironments Following Acute Exposure.
    Article Snippet: ROS was measured by flow cytometry (Attune NxT V6) using the indicator dye 20,70- dichlorodiydrofluorescein diacetate (H2DCFDA; Thermofisher Scientific [eg, 495 nm/em: 527 nm], which is reduced to 20,70- dichloroflurescein (DCF) in the presence of ROS, following the manufacturer’s protocol.

    Article Title: Inhalation of Tungsten Metal Particulates Alters the Lung and Bone Microenvironments Following Acute Exposure.
    Article Snippet: Cells were resuspended in 300 ml of staining buffer (1 dPBS, 10 mM sodium azide, 5% FBS) before being analyzed by flow cytometry (Attune NxT V6, Thermofisher Scientific).



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    flow cytometry attune nxt v6  (Thermo Fisher)
    90
    Thermo Fisher flow cytometry attune nxt v6
    Flow Cytometry Attune Nxt V6, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flow+cytometry+attune+nxt+v6/attune+nxt+flow+cytometer/pm38539048-96-4-9
    Average 90 stars, based on 1 article reviews
    flow cytometry attune nxt v6 - by Bioz Stars, 2026-09
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    attune nxt v6 flow cytometry  (Thermo Fisher)
    90
    Thermo Fisher attune nxt v6 flow cytometry
    Depletion of γδ T cells significantly decreases the severity of DED inflammation. ( A ) WT mice were administrated intraperitoneally with anti-γδ T-specific mAb or isotype control Ab 1 day before and 3 days after DED induction. ( B , C ) The depletion efficiency in mouse conjunctiva was confirmed on day 5 by flow <t>cytometry</t> ( n = 3–5). ( D ) Representative images of corneal fluorescein staining ( n = 10). ( E ) Changes in the corneal fluorescein staining scores. ( F ) Conjunctival tissue sections were stained with periodic acid Schiff (PAS) and the goblet cells were counted ( G ). ( H ) Quantitative PCR analysis for the expression of IL-17A, RORγt, NLRP3, IFN-γ, IL-1β, and IL-23 in the mouse conjunctiva. In the graph, each bar represents the mean ± standard error of the mean. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
    Attune Nxt V6 Flow Cytometry, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flow+cytometry+attune+nxt+v6/attune+nxt+flow+cytometer/pmc09652718-46-13-18
    Average 90 stars, based on 1 article reviews
    attune nxt v6 flow cytometry - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Depletion of γδ T cells significantly decreases the severity of DED inflammation. ( A ) WT mice were administrated intraperitoneally with anti-γδ T-specific mAb or isotype control Ab 1 day before and 3 days after DED induction. ( B , C ) The depletion efficiency in mouse conjunctiva was confirmed on day 5 by flow cytometry ( n = 3–5). ( D ) Representative images of corneal fluorescein staining ( n = 10). ( E ) Changes in the corneal fluorescein staining scores. ( F ) Conjunctival tissue sections were stained with periodic acid Schiff (PAS) and the goblet cells were counted ( G ). ( H ) Quantitative PCR analysis for the expression of IL-17A, RORγt, NLRP3, IFN-γ, IL-1β, and IL-23 in the mouse conjunctiva. In the graph, each bar represents the mean ± standard error of the mean. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Conjunctiva Resident γδ T Cells Expressed High Level of IL-17A and Promoted the Severity of Dry Eye

    doi: 10.1167/iovs.63.12.13

    Figure Lengend Snippet: Depletion of γδ T cells significantly decreases the severity of DED inflammation. ( A ) WT mice were administrated intraperitoneally with anti-γδ T-specific mAb or isotype control Ab 1 day before and 3 days after DED induction. ( B , C ) The depletion efficiency in mouse conjunctiva was confirmed on day 5 by flow cytometry ( n = 3–5). ( D ) Representative images of corneal fluorescein staining ( n = 10). ( E ) Changes in the corneal fluorescein staining scores. ( F ) Conjunctival tissue sections were stained with periodic acid Schiff (PAS) and the goblet cells were counted ( G ). ( H ) Quantitative PCR analysis for the expression of IL-17A, RORγt, NLRP3, IFN-γ, IL-1β, and IL-23 in the mouse conjunctiva. In the graph, each bar represents the mean ± standard error of the mean. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Article Snippet: Flow cytometry data were collected using the Cytoflex flow cytometry (Beckman Coulter) or Attune NxT V6 flow cytometry (ThermoFisher) and analyzed by FlowJo software (Tree Star Inc.).

    Techniques: Control, Flow Cytometry, Staining, Real-time Polymerase Chain Reaction, Expressing

    γδ T cells were identified based on positive expression of γδ T cell receptor (TCRγδ). ( A ) Gating strategy and representative flow cytometry plots for analyzing γδ T cells on the human ocular surface. The γδ T cells were gated on live cells and live CD45 + cells, respectively. ( B , C ) The quantitative summary of γδ T-cell proportion among live cells, with ( B ) or without ( C ) the 2 outliners. ( D , E ) The quantitative summary of γδ T-cell proportion among CD45 + cells, with ( D ) or without ( E ) the 2 outliners. ( F ) The quantity in the human ocular surface. In the graph, each bar represents the mean ± standard error of the mean. * P < 0.05. n = 11 for patients with mild DED, and n = 11 for patients with severe DED.

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Conjunctiva Resident γδ T Cells Expressed High Level of IL-17A and Promoted the Severity of Dry Eye

    doi: 10.1167/iovs.63.12.13

    Figure Lengend Snippet: γδ T cells were identified based on positive expression of γδ T cell receptor (TCRγδ). ( A ) Gating strategy and representative flow cytometry plots for analyzing γδ T cells on the human ocular surface. The γδ T cells were gated on live cells and live CD45 + cells, respectively. ( B , C ) The quantitative summary of γδ T-cell proportion among live cells, with ( B ) or without ( C ) the 2 outliners. ( D , E ) The quantitative summary of γδ T-cell proportion among CD45 + cells, with ( D ) or without ( E ) the 2 outliners. ( F ) The quantity in the human ocular surface. In the graph, each bar represents the mean ± standard error of the mean. * P < 0.05. n = 11 for patients with mild DED, and n = 11 for patients with severe DED.

    Article Snippet: Flow cytometry data were collected using the Cytoflex flow cytometry (Beckman Coulter) or Attune NxT V6 flow cytometry (ThermoFisher) and analyzed by FlowJo software (Tree Star Inc.).

    Techniques: Expressing, Flow Cytometry

    The γδ T cells are the main producers of IL-17A in the DED mouse conjunctiva. ( A , B ) Representative flow cytometry plots and quantitative summary of CD45 + cells, γδ T cells, CD4 + T cells, and CD8 + T-cell percentage among the conjunctiva live cells. ( C , D ) Representative flow cytometry plots and quantitative summary of γδ T cells, CD4 + T cells, and CD8 + T cells in the conjunctiva of the mice in the control group (N) and the DED group. ( E – H ) The conjunctival cells were stimulated with PMA plus ionomycin for 4 hours in the presence of brefeldin A (BFA). The cells were stained with mAb against surface markers of each T cell subset and innate lymphoid cells, then intracellularly with anti–IL-17A mAb and analyzed by flow cytometry. Representative flow cytometry plots and proportions of IL-17A–producing ILCs among CD45 + lymphoid cells are shown in ( E ) and ( F ). IL-17A + Lin + cell populations from conjunctiva were further analyzed for TCRγδ, CD4, CD8a, and NK1.1 expression by flow cytometry ( G ). The proportion of IL-17A–producing cell subsets (γδ T, CD4 + T, CD8 + T, NK T cells, and ILCs cells) among CD45 + cells in the control and DED groups are shown in ( H ). ( I , J ) The representative FACS plots and summary of the Vγ4 subset of IL-17A–producing γδT cells in the conjunctiva. Data represent the mean ± standard error of the mean ( n = 10–12). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Conjunctiva Resident γδ T Cells Expressed High Level of IL-17A and Promoted the Severity of Dry Eye

    doi: 10.1167/iovs.63.12.13

    Figure Lengend Snippet: The γδ T cells are the main producers of IL-17A in the DED mouse conjunctiva. ( A , B ) Representative flow cytometry plots and quantitative summary of CD45 + cells, γδ T cells, CD4 + T cells, and CD8 + T-cell percentage among the conjunctiva live cells. ( C , D ) Representative flow cytometry plots and quantitative summary of γδ T cells, CD4 + T cells, and CD8 + T cells in the conjunctiva of the mice in the control group (N) and the DED group. ( E – H ) The conjunctival cells were stimulated with PMA plus ionomycin for 4 hours in the presence of brefeldin A (BFA). The cells were stained with mAb against surface markers of each T cell subset and innate lymphoid cells, then intracellularly with anti–IL-17A mAb and analyzed by flow cytometry. Representative flow cytometry plots and proportions of IL-17A–producing ILCs among CD45 + lymphoid cells are shown in ( E ) and ( F ). IL-17A + Lin + cell populations from conjunctiva were further analyzed for TCRγδ, CD4, CD8a, and NK1.1 expression by flow cytometry ( G ). The proportion of IL-17A–producing cell subsets (γδ T, CD4 + T, CD8 + T, NK T cells, and ILCs cells) among CD45 + cells in the control and DED groups are shown in ( H ). ( I , J ) The representative FACS plots and summary of the Vγ4 subset of IL-17A–producing γδT cells in the conjunctiva. Data represent the mean ± standard error of the mean ( n = 10–12). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Article Snippet: Flow cytometry data were collected using the Cytoflex flow cytometry (Beckman Coulter) or Attune NxT V6 flow cytometry (ThermoFisher) and analyzed by FlowJo software (Tree Star Inc.).

    Techniques: Flow Cytometry, Control, Staining, Expressing